Sedimentation Equilibrium Studies on Protein From Kookaburra Beak
نویسندگان
چکیده
منابع مشابه
Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity.
Regulated molecular interactions are essential for cellular function and viability, and both homo- and hetero-interactions between all types of biomolecules play important cellular roles. This chapter focuses on interactions between membrane proteins. Knowing both the stoichiometries and stabilities of these interactions in hydrophobic environments is a prerequisite for understanding how this c...
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Determination of the assembly state(s) of a membrane protein poses a difficult analytical problem. A powerful technique that is well adapted to meet this challenge is solution interaction analysis using analytical ultracentrifugation. Due to their hydrophobic nature, membrane proteins require detergent (or lipid) for solubilization in solution. Thus, a fundamental obstacle to be overcome in det...
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In 1957, Meselson, Stahl, and Vinograd 1 introduced the remarkable technique of sedimentation equilibriuln in a density gradient for the study of the properties of high molecular weight DNA in solution. Because of the very high molecular weights of DNA, conventional sedimentation equilibrium cannot be used; for even at the lowest centrifugal fields which are practical, the force on these molecu...
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ژورنال
عنوان ژورنال: Australian Journal of Biological Sciences
سال: 1976
ISSN: 0004-9417
DOI: 10.1071/bi9760481